Dear Ed -
I agree with you, and I felt a little funny giving that advice, since I
know that Rmerge is rather outdated as a resolution cutoff. What I was
really reacting to was the slope of I versus resolution; if you look at
Qing's Scalepack table, you can see that average I plateaus in the low 4
A range, and I usually get worried about the data when I don't see a
steady decrease in I with increasing resolution.
Having said that, I don't usually work with datasets at this resolution,
and it's possible (even likely?) that I was fooled by the bump in the
Wilson plot around 4. Perhaps this data does extend to 4.1.
- Matt
On 9/7/12 11:39 AM, Edwin Pozharski wrote:
Matt,
On 09/07/2012 09:56 AM, Matthew Franklin wrote:
I'm also a bit dubious about the 4.3 A limit; your useful data may be
ending around 4.6 instead, despite the high I/sigma numbers.
Why? I would rather suggest Qing extends resolution to where
I/sigma~1. Other than Rmerge, I don't see what else you may be
looking at, and that is "traditional but inferior" way to determine
resolution cutoff.
Cheers,
Ed.
--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374
