Hi Rashmi, I can't say that we use it regularly. There are only one or two protocols in our lab that need it in the buffers. I didn't do the actual work so I am not sure if there was any problem caused by it regarding the protein purification or functionality. But those protocols are quite established for the specific proteins. I suppose that supports the idea that it at the minimum does not always cause problems.
Zhijie From: Rashmi Panigrahi Sent: Thursday, September 20, 2012 8:29 AM To: Zhijie Li Cc: [email protected] Subject: Re: [ccp4bb] sarkosyl Thanks Zhijie, So do you mean to say that your lab regularly uses it in Lysis and refolding buffers, and the protein/s purified are structurally and functionally fine and that there is no aggregation caused due to sarkosyl?? kind regards Rashmi On Thu, Sep 20, 2012 at 6:23 PM, Zhijie Li <[email protected]> wrote: Hi, We used it in some lysis/refolding buffers. Sigma marked this biodegradable detergent as "Highly toxic by inhalation" - when it's used in toothpastes and shampoos if I am not mistaken. when I called, Sigma said that since someone published a paper saying that when they sprayed it into a rat's nose the rat died, they had no choice but to put a skull sign on the MSDS. Zhijie From: Rashmi Panigrahi Sent: Thursday, September 20, 2012 5:47 AM To: [email protected] Subject: [ccp4bb] sarkosyl Hi All, I was wondering if anyone has used N-lauryl sarcosine in the lysis buffer for purifying protein and checked if the protein was functional or structurally okay Please let me know kind regards -- rashmi -- rashmi
