On 09/24/2012 07:09 AM, Harm Otten wrote:
Why is it so hard to break the symmetry for two (seemingly) different monomers?

Because it's present in your data?

In P1, do you still have the electron density that suggests the "overlap" (it's not entirely clear to me what you mean by that - a figure could help)?

Twinning?

An outside chance that you have a mix of the full-length and N-terminal truncation?

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