Peter We make a 96-well plate that was originally made for microbatch-under-oil, which has a large "moat" or well around the whole plate.
My old friend Lesley Haire had a lot of success with this plate for a condition that contained isopropanol. She set up the drops without isoprop, covered them with Al's Oils (silicone mixed with paraffin oil from Hampton Research), then put water containing the appropriate concentration of isoprop in the moat (in her case 10 - 20%). THe isoprop diffused through the oil and into the drops. Since the oil is also saturated with isoprop it makes a very good barrier and stops the isoprop from evaporating from the drops when you harvest your crystals. As Lesley pointed out you can even set up a random screen without isopropanol, then diffuse the isopropanol in afterwards. Let me know if you - or anyone else - would like some samples of the plate. Unfortunately it won't fit on a Mosquito, but you can use it by hand, see ref below. Regarding your question below, bear in mind that water and oil can both go through plastic tubes (slowly). I hope it works for you Patrick Refs and info: See http://www.douglas.co.uk/winner1.htm http://www.douglas.co.uk/vb.htm *Nature* *431* (2004), pp 481-485. On 3 October 2012 17:37, Peter Hsu <hsuu...@u.washington.edu> wrote: > Hi all, > > I recently got some hits from a Mosquito grown at 18C with PEG4000 and 10% > propanol. I've tried reproducing these hits using the standard 24 well > format with no success, even with playing with PEG and propanol > concentrations. The initial screening solution was from a kit that's been > sitting in the cold room for the better part of 2 years. Does anyone here > have any tips or tricks in reproducing crystals grown from organic solvents? > > Many thanks in advance, > > Peter > -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
