since you have already identified the correct beam position through the ice rings I would fix that beam position. My assumption is that the drifting of your predicted spots is due to "shifts" in the beam position. Also you should probably fix the detector distance at that resolution.
Jürgen On Oct 18, 2012, at 3:15 PM, Jan van Agthoven wrote: Hi everyone, I recently switched from HKL2000 to imosflm to get rid of ice rings. The group space and cell unit of the data set are known and perfectly recognized by HKL2000. The predictions are also correct. In imosflm, the unit cell and space group are recognized. However the predictions are terrible, and it even get worse after cell refinenement. I tried to use different images, different resolution range (my data set is at 3.3 A), I placed the beam center using an ice ring. Nothing works. Imosflm also does not seem to allow changes to its defaullt parameters. Does anyone have an idea what I can do, my main goal being to get rid of ice rings in a 3.3 A data set? Thanks, ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
