Hello everybody,
I collected datasets with a resolution to 2.8A from 3 crystal grown in the
same condition. The space group seems to be P63. Statistic of XSCALE.LP
SUBSET OF INTENSITY DATA WITH SIGNAL/NOISE >= -3.0 AS FUNCTION OF RESOLUTION
RESOLUTION NUMBER OF REFLECTIONS COMPLETENESS R-FACTOR R-FACTOR
COMPARED I/SIGMA R-meas CC(1/2) Anomal SigAno Nano
LIMIT OBSERVED UNIQUE POSSIBLE OF DATA observed expected
Corr
10.00 1023 173 204 84.8% 7.4% 14.1%
1023 10.86 8.1% 99.5* -28 0.422 156
6.00 4199 704 709 99.3% 11.1% 15.1%
4199 10.43 12.1% 99.1* -25 0.515 640
4.00 12401 2131 2137 99.7% 15.5% 15.5%
12400 9.91 17.0% 97.7* -18 0.684 1893
3.50 8429 1486 1486 100.0% 18.3% 18.0%
8426 8.10 20.1% 96.4* -8 0.788 1259
3.30 4630 842 842 100.0% 23.0% 22.3%
4627 6.59 25.5% 94.5* -13 0.768 686
3.10 6057 1121 1121 100.0% 28.3% 26.3%
6053 5.50 31.4% 93.6* -3 0.829 882
3.00 3527 664 664 100.0% 33.7% 34.2%
3524 4.56 37.5% 86.8* 6 0.827 502
2.90 3961 756 756 100.0% 48.4% 49.5%
3956 3.24 53.8% 82.7* 2 0.784 549
2.80 2140 699 862 81.1% 31.6% 37.4%
2057 2.51 37.7% 87.0* 2 0.789 178
total 46367 8576 8781 97.7% 17.2% 18.1%
46265 7.15 19.0% 98.2* -9 0.730 6745
I tried Xtriage and got the following:
merohedral twin operator
twin law: h,-h-k,-l
Britton plot: 0.423
H-test: 0.439
Maximum Likelihood Method: 0.457
I have a model with the sequence of my protein and did Phaser_MR (Z-score
around 4... I know that this is quiet bad...). This solution I refined with
phenix.refinement using the twin law from Xtriage. Rfree is around 0.44 best
but the model looks weird...
Greetings,
Iris
___________________________________
Iris Gawarzewski
PhD student
Arbeitskreis Schmitt
Institut für Biochemie
Geb. 26.32.03.21
Heinrich-Heine-Universität Düsseldorf
Universitätsstr. 1
40225 Düsseldorf
-Germany-
Tel: 0049-211-81-13577
