Put me down at another person who re-suspends bacterial cell pellets in 4-5 volumes of buffer.
On Thu, Oct 25, 2012 at 9:15 AM, Opher Gileadi <[email protected]>wrote: > It makes sense to use a fixed ratio of resuspension buffer to cell weight; > we weigh the pellets after centrifugation, then suspend in at least 4-5 > volumes (ml/gr) of buffer. >
