Dear Karolina, We had one nice example where the protein could easily be concentrated to about 50mg/mL without crystallising, at which point we realised that it would be a good project for collaborating with our NMR colleagues, i.e. the RING domain of the Kaposi's sarcoma-associated herpesvirus K3 protein (Dodd et al., JBC 279:53840-53847, 2004). I wonder if you'd find other examples by looking at NMR structures -- unless, of course, you're after proteins of still unknown structure!
Best wishes, Randy ----- Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: +44 1223 336500 Wellcome Trust/MRC Building Fax: +44 1223 336827 Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk On 24 Oct 2012, at 22:47, Karolina Michalska wrote: > Hello everybody, > > I'm looking for small proteins (up to 150 aa) that are known to express > reasonably in E. coli, are soluble and folded but failed to crystallize. > Can you provide some examples, especially of proteins that might be > interesting for a broad community? > > Thanks, > Karolina
