Yes. It's not different from two alternate conformations of the same residue as
far as refmac is concerned. Just name one AXXX, the other BYYY and give them
each occupancies less than 1. Refmac5 will handle them properly.
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: [email protected]
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
________________________________________
From: CCP4 bulletin board [[email protected]] on behalf of Fred Kerff
[[email protected]]
Sent: Monday, October 29, 2012 3:58 PM
To: [email protected]
Subject: [ccp4bb] Different residue type with same number in refmac?
Hello,
The protein preparation I'm working with is not homogeneous. It comes from a
natural source and few amino acid are not conserved. Is there a way to take
this into account in refmac5. In other words having two alternative
conformations with two different residue names?
Thanks for your help.
Fred
-----
Frédéric Kerff
Chercheur qualifié F.R.S.-FNRS
Cristallographie des protéines
Centre d'Ingénierie des Protéines
Université de Liège
17, Allée du 6 Août - Bat B5a
4000 Liège (Belgium)
Tel.: +32 (0)4 3663620
Fax: +32 (0)4 3663772
