Hi Uli, I think Proteinase K and PMSF should work, though I never tried.
Cheers, Dmitry On 2012-11-09, at 9:12 AM, Ulrich Zander wrote: > Hi All, > > > For a tutorial, we would like to demonstrate the method of ligand soaking in > protein crystals. I am thinking about using some sort of native ligand or > inhibitor that can be easily identified in the electron density rather than > halide or heavy atom derivatization. > > Does anybody have a suggestion for a protein/ligand combination that could be > used for that and that is commercially available? > > > Thanks! > > Uli > > > > > > > > > -- > -------------------------------------------------------------------- > Ulrich Zander > High Troughput Crystallization Lab/Diffraction Instrumentation Team > EMBL Grenoble Outstation > 6 rue Jules Horowitz > 38000 Grenoble, France > Phone: +33 47620-9487 > --------------------------------------------------------------------
