Dear all - we have a synchrotron trip coming up and have crystals that
are likely to contain intrinsically bound zinc atoms. The crystals
diffract to 4A-ish so far. We're hoping to collect both native datasets
at higher dose, and phasing datasets at lower dose (on separate
crystals). Can we do the lot at the zinc edge wavelength, or should we
be worried about radiation damage, and move away from the edge for the
native datasets? And would a native dataset for refinement be
compromised by friedel pair differences collected at an edge?
Alan
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Alan Cheung
Gene Center
Ludwig-Maximilians-University
Feodor-Lynen-Str. 25
81377 Munich
Germany
Phone: +49-89-2180-76845
Fax: +49-89-2180-76999
E-mail: [email protected]