These look like spherulites. They are often optimizable into crystals by
varying the pH, precipitant/protein concentration,
additives,crystallization temperature, etc.
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [email protected]
On 12/1/2012 8:24 PM, Yibin Lin wrote:
Dear Folks,
I got some crystals at the conditions of 0.1M Lithium sulfate or 0.1 M
MgCl2, 0.1M Sodium citrate 3.5 or Sodium acetic ph5.5, or 0.1M HEPES
pH7.5, or 0.1M Tris pH8.5, 30% PEG400 or 12% PEG4000. Protein contains
0.017%DDM.
Could anyone have the experience to say if it is protein crystal? And
how to optimize these crystals.
Thanks.
Lin
