Respected members,,
I have one question bearing in my
mind regarding soaking. Binding of the substrate to enzyme leads to active
site closure. So if we are soaking the enzyme crystal with the substrate,
and collecting the data and solving the structure, then how would one
distinguish the existence of apo and substrate bound form of enzyme in same
crystal. I meant to say, soaking may cause the crystal to have quasi-apo-enzyme
and quasi-substrate-enzyme complex existing together. Is there any way to
take care of such pathological condition during data processing, or
structure solving? . I would highly appreciate your help and looking
forward to get valuable advices. Please graduate me in this kind of weired
situation.
Thank you
Appu
