Joy,
Try 80% saturated Lithium sulfate (2.5M) instead.
Just transfer the crystals and if it does not work
let me have more precise crystallization conditions and I will post you
other options.
Enrico.
On Thu, 03 Jan 2013 18:33:05 +0100, Joy <[email protected]> wrote:
Dear All,
May I have your expert opinion on the following question: what is the
minimum concentration of ammonium dibasic phosphate if I would like to
use
it as cryoprotectant?
Happy New Year!
Joy
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [email protected] Fax: 33 (0)1 69 08 90 71
