Hello, Does anyone have any experience working with acidic bicelles for crystallization of membrane proteins?
I am working with a small (~100 amino acid), trimeric protein that is not a membrane protein itself but is only fully folded when in the presence of an anionic membrane mimetic, such as PC:PA liposomes or SDS. I would like to try crystallization with DMPC:DHPC bicelles spiked with DMPA but cannot find a good protocol for making this type of bicelle. I have tried adapting the protocol from JOVE by Ujwal and Abramson with no success. Also, what would be a good q value to start with, given that I do not know anything about the footprint of my protein? From what I have read, small q ratios (~1) seem to be good for high resolution structure work, but this comes mostly from the NMR field. Thanks! Jessica Jessica Silverman Molecular Microbiology PhD Candidate Heldwein Laboratory, A611 Tufts University School of Medicine 136 Harrison Ave., Boston, MA phone: 617-636-0474 email: [email protected]
