Hi all, Sorry for the incredibly dumb and off topic question. I've been doing some steady state michaelis menten kinetics, and no one around me has ever done it before. My own classroom training was years ago and so I've forgotten a great deal and have just been going from old textbooks/papers.
So my assay is so far fixed [enzyme] and varied substrate, and I do my reaction for an hour, and then I get a concentration of product produced from UV. Is my initial velocity at each concentration point just product(uM)/90 minutes/60 seconds? I'm just doubting my work only because my calculated Kcat's are somewhere in the range of 0.0001 s-1, which just seems incredibly off especially when compared to other enzymes. Thanks and sorry again for the off topic question. Peter
