Does anybody have a nice, cite-able reference for the observation that GST tags can sometimes drag otherwise-aggregated/unfolding/generally unhappy proteins into solution?
We need to politely explain why a His-tagged version of our protein (purified in my lab) has activity but a GST-tagged version (purified in a different lab) doesn't. thanks, Phoebe ++++++++++++++++++++++++++++++++++++++++++ Phoebe A. Rice Dept. of Biochemistry & Molecular Biology The University of Chicago 773 834 1723; [email protected]<mailto:[email protected]> http://bmb.bsd.uchicago.edu/Faculty_and_Research/ http://www.rsc.org/shop/books/2008/9780854042722.asp
