I guess you may have to decide whether you want to modify your current conditions or just start over to find new conditions, to get around this "ultra-fast" crystallization. I don't know if you have played around all parameters of current condition, such as lower temperature, protein concentration, pH, salt, etc, or ways to slow down vapor diffusion, i.e. covering drop or reservoir by silicon oil. If all these do not help, you might have to find a new condition under which protein crystallizes in a different morphology...However, BEFORE you try out things at the crystallization stage, ask if there is anything that you could improve before crystallization: Is your construct good enough? How is your protein quality--purity/homogeneity? Fine-tuning every step from gene to protein to crystal could eventually lead to good results.
Joe On Tue, Jan 15, 2013 at 8:30 PM, Mike John <[email protected]> wrote: > Hello, all, > > My crystal grows very fast even though the protein conc. is now 1.5 mg/ml. > The shape of the crystal likes a ruler plate with one side very thin. The > crystal quality has diffraction to about 3.5A in a home source of 1.2 KW > sealed tube Angilent equipment. But the data can not be indexed due to one > direction has poor diffraction and the crystal quality. Seeking advices on > improving the crystal quality. Thank you very much > > Mike > > -- Best regards, Joe
