Hi Theresa, The overexpression of any MP may depend on 100 factors and what you listed below are just a few!
toufic On Thu, Jan 17, 2013 at 9:50 PM, Theresa Hsu <[email protected]> wrote: > Dear all > > I have a general question on membrane protein overexpression in E. coli. > Are E. coli proteins expressed in E. coli always better in terms of yield, > lipid preservation and so on than another homologous protein? > > I am aware that it is different for *crystallization* because of flexible > loops and thermostability. > > Thank you. > > Theresa > >
