Indeed there are many web tools predicting solubility. My personal bias is that your brain is the best tool for ideas on how to design expression constructs. (since one question in a multi-domain protein, is which bit is interesting ...!) Many brains are better than one (talk to your colleagues - even to your supervisor ! - for ideas).
A tool we like (since we developed it ...) helps to combine information from many other tools and help us make decisions (and order pcr primers to implement our decisions) is available at: http://xtal.nki.nl/ccd Another advice is whatever constructs you try, try them in many vectors - I could give another self-plugin here but I will not. There are many good systems allowing to put the same PCR product to many vectors for expression. A. > From: Careina Edgooms <careinaedgo...@yahoo.com> > Reply-To: Careina Edgooms <careinaedgo...@yahoo.com> > Date: Tue, 22 Jan 2013 04:22:44 -0800 > To: <CCP4BB@JISCMAIL.AC.UK> > Subject: [ccp4bb] protein solubility predictions > > Dear ccp4 > > Apologies for the off topic question. I was wondering whether anyone could > suggest a good tool or methodology to use to predict protein solubility and > ability to fold from the sequence? I am working with a large protein of > multiple domains. I would like to work with as close to the full length > protein as possible without affecting its solubility and ability to fold > correctly. I know there are web based tools where you can upload a sequence > and see the predicted solubility but I wonder if there is any good strategy > to use to determine how best to construct the truncated protein? ie which > parts of the sequence to keep and which to remove so as to maximise > solubility. Also I have an eye to crystallising this protein in the future > and I wonder if there are any specific things I should look out for with that > in mind? I'm sure minimising flexible loops is one such thing. > All help appreciated > Best > Careina
