There a lot of articles about salt-protein crystals in google - check
them first.
How to check crystals:
1) X-ray check (most obvious way)
2) izid dye
3)dry it (protein crystal will break apart)
4)crush it (if you dont know how to check by this method - try to grow
and break lysozyme or another model protein crystals - it will take not
more than 3 days) . Protein crystals behave rather as gelatine and not
as solid
08.02.2013 13:53, Sylvia Fanucchi пишет:
I didn’t see the picture that you attached but if you have more than
one crystal you could always run one on a gel to see if it runs the
expected size of your protein
------------------------------------------------------------------------
*From:*Patrick Shaw Stewart [mailto:[email protected]]
*Sent:* Friday, February 08, 2013 11:47 AM
*To:* [email protected]
*Subject:* Re: [ccp4bb] protein crystals or salt crystals
Good morning Frank
On a related idea, do you typically use a limited number of "buffers"
(buffer plus salt) for the final purification step of your proteins?
If so, do you have a chart of where salt crystals may appear in the
screens that you use most often? Could you put that chart on your web
site to help the community?
People could pick one of your standard buffer mixes to make their
lives easier later on.
Best wishes
Patrick
On 8 February 2013 07:18, Frank von Delft <[email protected]
<mailto:[email protected]>> wrote:
Test the diffraction - that's the only way. But given the other junk
in the drop, chances are they're salt.
(And don't post 5Mb attachments, please.)
On 07/02/2013 22:24, amro selem wrote:
Hallo my colleagues.
i hope every one doing ok . i did screening since two weeks . i
noticed today this crystals. i don`t know either it salt or protein
crystal . my protein has zero tryptophan so i could distinguish by UV
camera.
the condition was conditions:
0.1M SPG buffer pH 8 and 25%PEG 1500. in addition to Nickle chlorid 1mM.
best regards
Amr
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