Dear Mark, we have had a slight success increasing the solubility of some of our proteins adding high concentration of salt (in addition to betaine and glucose) to the culture media, as described in this paper
http://www.ncbi.nlm.nih.gov/pubmed/17126029 Sergio 2013/2/26 Mark J van Raaij <[email protected]> > Dear All, > > We are considering trying additives to bacterial cultures to see if they > improve solubility of some proteins we express: L-arginine, glycyl-glycine, > sorbitol and betaine. I would be glad to receive recommendations and > experiences, also if they haven't worked. > Side-question: should we get cell culture-certified compounds, or would > "normal", much more economical ones suffice? (i.e. are they likely to > contain side-products that would affect bacterial growth?). > > Greetings, > > Mark > > Mark J van Raaij > Lab 20B > Dpto de Estructura de Macromoleculas > Centro Nacional de Biotecnologia - CSIC > c/Darwin 3 > E-28049 Madrid, Spain > tel. (+34) 91 585 4616 > http://www.cnb.csic.es/~mjvanraaij >
