Along these lines, what reagents do people use to promote disuflide bonds,
i.e., the "anti-DTT?"
Glutathione (red) + Glutathione (ox), redox potential is adjusted by
varying the ratio.
Best,
Clemens
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs
<[email protected]>wrote:
You might want to try "Disulfide by design"
http://cptweb.cpt.wayne.edu/DbD2/
Cheers
Dave
On Feb 28, 2013 6:55 AM, "Careina Edgooms" <[email protected]>
wrote:
Dear CCP4 members
I wish to engineer a disulfide bond at the dimer interface of a protein I
am working with. Does anyone know of any available software to assist with
this?
Best
Careina
--
*******************************************
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: [email protected]
*******************************************
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Dr. Clemens Grimm
Institut für Biochemie
Biozentrum der Universität Würzburg
Am Hubland
D-97074 Würzburg
Germany
e-mail: [email protected]
phone : +49 0931 31 84031
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