Hi All,
Really sorry for my non-CCP4 related question. Our lab mainly uses
bacterial cultures to produce targets proteins for crystallizations.
However, we have been struggling with phage infections to our
bacterial cultures for a quite long time. To control its devastating
effects, we have regularly been using large-dose ozone treatments on
the whole lab space. We have also tried anti-phage BL21/DE3 strains
from Sigma USA but found it was still not avoidable. The lab has been
maintained well hygienic and its outdoor environment is clean and
neat. We keep good ventilations, including windows and central AC
systems. However, phages are still eating up our cultures with very
low percentage of survivals. Therefore, this has been our big
headache. We wonder whether you have the same experience and how to
keep a lab free of those bugs. Your suggestions are deeply
appreciated. Thanks.
Sincerely
Aidong
Aidong Han, Ph.D
Department of Biomedical Sciences
School of Life Sciences
Xiamen University
3 South Xiangan Road
Xiangan, Xiamen 361102
China
Phone: 0592-218-8172 (O)
0592-218-8173 (L)
Web: http://life.xmu.edu.cn/adhanlab/
