Hi guys, I need to manually build a model in Coot without a map. I want to link to 2 loops from 2 DNA strands together by base-pairings while keep the rest part as freezing as possible. The sequences of the loops are complimentary but the current orientations of the loops is not correct. Is there a way to add restraint between the loops to force them base-pairing while keep the rest part of the 2 DNA strands fixed?
Another question is how to measure the angles between two DNA helices? I have seen a command to measure protein helices in PYMOL but did not find one for DNA helices. Thanks for your great help! Cheers, Frank
