Hi Mark, BLR(DE3) should be what you are after.
http://www.emdmillipore.com/life-science-research/blrde3-competent-cells/EMD_BIO-69053/p_yYub.s1Ol18AAAEjORl9.zLX hth, Marko On Wed, 27 Mar 2013, Mark J van Raaij wrote:
Dear All, we were wondering if knows of an Escherichia coli B strain that is Rec deficient (Rec-). We want to compare certain properties of E coli B and K12, and for the experiment it would be best to use a Rec- variant. We have TOP10, which is a K12 derivative that is Rec-, but derivatives of B like BL21 are Rec+. Have a happy Easter, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij
_____________________________________ Marko Hyvonen Department of Biochemistry, University of Cambridge [email protected] http://www-cryst.bioc.cam.ac.uk/groups/hyvonen tel: +44-(0)1223-766 044 mobile: +44-(0)7796-174 877 fax: +44-(0)1223-766 002 --------------------------------------
