Dear Tommi, We have been very successful with large scale transient transfections of suspension grown HEK293F cells. See recent paper for methods: Nature 2012 481:335-40. Has worked well for all complexes we have tried with only one exception. Happy to answer any Q's ([email protected]). Louise
On Fri, Mar 29, 2013 at 12:01 AM, Tommi Kajander <[email protected] > wrote: > Dear all, > > I would be interested in comments/opinions about what is your experience > on which > would be the best mammalian cell culture vector/cell line/medium > combination for transient > expression to get the best yields. Depends, of course, but anyway > obviously there are > differences, HEKs are suppose to be easy to transfect, but which flavour > and how and > what medium or would you prefer CHO? (probably more so for stable cell > lines?) > > ...etc. vectors...? Or does it make a difference (it seems to). Good > reviews/comparisons? > is it worth going beyond DMEM and supplements? (what do you add in?) > > Stupid questions - trying to educate myself a bit here.. and i am willing > to spend a bit or > at least know what difference it makes (time is money anyway ...and worse). > > and we are not doing suspension at the moment, but could consider it if it > seems like a > good idea. > > Thanks for the tips.., > Tommi > > > > > > >
