Dear Careina,
I would be cautious of using dyes. Much better to 1) try in-situ diffraction if possible as this is least invasive or 2) pick a sensible cryo and just freeze the crystal(s). I would try to same something from the experiment for seed stock & possibly sequencing. Dave David Hargreaves Associate Principal Scientist _____________________________________________________________________ AstraZeneca Discovery Sciences, Structure & Biophysics Mereside, 50F49, Alderley Park, Cheshire, SK10 4TF Tel +44 (0)01625 518521 Fax +44 (0) 1625 232693 David.Hargreaves @astrazeneca.com <mailto:name.surn...@astrazeneca.com> Please consider the environment before printing this e-mail From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Careina Edgooms Sent: 15 April 2013 11:18 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] salt or not? Dear ccp4 I have been performing trials on a protein DNA complex for a while now and have not seen any crystals form. Today I checked an old plate (over a month old) and I see 4 large crystals. *excitement* Three of them look tetragonal in shape (like a pyramid) and one of them looks hexagonal. I do not know if they are salt or protein. There is calcium chloride in the buffer. They feel quite soft to touch. They do not cause much birefringence. One of them does not seem to absorb much izit. It did go a bit blue but not entirely. How can I tell if this crystal is protein or not? Do you think its worth trying to see how it diffracts? Also, does Izit affect diffraction/ protein structures at all? Could I use a crystal with Izit in a diffraction experiment and ultimately to get the structure? Best Careina -------------------------------------------------------------------------- AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 2 Kingdom Street, London, W2 6BD. Confidentiality Notice: This message is private and may contain confidential, proprietary and legally privileged information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorised use or disclosure of the contents of this message is not permitted and may be unlawful. Disclaimer: Email messages may be subject to delays, interception, non-delivery and unauthorised alterations. Therefore, information expressed in this message is not given or endorsed by AstraZeneca UK Limited unless otherwise notified by an authorised representative independent of this message. No contractual relationship is created by this message by any person unless specifically indicated by agreement in writing other than email. Monitoring: AstraZeneca UK Limited may monitor email traffic data and content for the purposes of the prevention and detection of crime, ensuring the security of our computer systems and checking Compliance with our Code of Conduct and Policies.
