P.S.: The C5 is what we had in Bochum. Also not perfect, but I guess theres no perfect machine ..
Von: Clemens Steegborn [mailto:clemens.steegb...@ruhr-uni-bochum.de] Gesendet: Donnerstag, 28. März 2013 13:58 An: 'Clemens Steegborn'; 'CCP4BB@JISCMAIL.AC.UK' Betreff: AW: [ccp4bb] cell disruptor / homogenizer - final summary Since my previous summary on cell disruption equipment elicited a pretty clear response, I want to give a final update: Several methods and machines were mentioned (see below), but the Avestin Emulsiflex C5 is now the clear winner, with five happy users and no really negative comment. Thanks again to everyone for this helpful feedback! Happy Holidays Clemens Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Clemens Steegborn Gesendet: Dienstag, 26. März 2013 19:21 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] AW: [ccp4bb] cell disruptor / homogenizer - summary Dear colleagues, To summarize the feedback on cell disruptors: One person found the Emulsiflex performance worth the maintenance required, there were no comments at all on Fluidizer and TS 0.75! As alternatives, French press (Glenn Milles), beadbeaters (Biospec), Panda" homegenizer (GEA Niro Soavi), and nitrogen cavitation in a pressure bomb (http://www.ncbi.nlm.nih.gov/pubmed/21041386) were suggested. Thanks for the comments and suggestions. Best Clemens Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Clemens Steegborn Gesendet: Montag, 25. März 2013 10:17 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] cell disruptor / homogenizer Dear colleagues, We are currently looking for new cell disruptor/homogenizer equipment, mainly for E.coli work. We currently have a Branson sonifier and a Microfluidics Fluidizer the latter one keeps causing trouble, and we think about a replacement. We previously also used an Avestin C-5 Emulsiflex, required quite some maintenance, and I inquired about a Constant Systems TS 0.75 and got mixed responses from users (not to mention their outrageous pricing). My question: What is your experience with Fluidizer, Emulsiflex, TS 0.75? Is there any other great (low maintenance, affordable especially concerning follow-up costs: repairs, replacement parts) equipment I missed? Thanks in advance for your comments Best regards Clemens
