Hi All: I wish to identify and remove unstructured regions at the C-terminus of my protein for crystallization and am considering using carboxypeptidase digestions to achieve this.
1) I would like a protocol and your preferred source of commercial carboxypeptidases as well their storage conditions. 2) Do I need to use a mixture of different carboxypeptidases? If so, which ones? 3) Is ESI-MS intact mass analysis a good way to identify the post-treatment C-terminus or does this treatment typically yield a large mixture of C-termini? 4) Any published references to this approach would be appreciated. Thank you. Best, Vish
