Hi, You can have a look at the paper titled "The funnel approach to the pre-crystallization production of membrane proteins" by Oded Lewinson, Allen T. Lee, and Douglas C. Rees. They have done high throughput over-expression of various membrane proteins.
-- Nazia Nasir PhD Scholar Protein Crystallography Lab National Institute of Immunology New Delhi On Sun, Apr 28, 2013 at 10:56 PM, Theresa Hsu <[email protected]> wrote: > Dear all > > I want to try a factorial approach for my His-tagged membrane proteins. > The screening parameters are E. coli strains, temperature and media. Is > there any simple but representative method I can use to compare the > expression level without isolating the membrane? Is whole cell lysis and > Western blot a good method? > > Thank you. > > Theresa >
