Hi Faisal, whatever makes your protein happy is good. What type of experiment were you thinking of ? Protein-protein interaction or protein-small molecule interaction ? In the latter case add some Tween20 or Brij-35 into the mix. A decent overview can be found under this webpage for what to consider when running your experiment. http://www.sprpages.nl Since you are on this board, my assumption is you have crystallography-grade protein, which is an excellent starting point for SPR experiments. One thing to consider though is to perhaps include a glycerol correction, similar like a DMSO calibration curve, but that very much depends on what type of experiment you want to pursue.
Feel free to send me details off-board, Jürgen ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu On May 5, 2013, at 5:07 PM, Faisal Tarique wrote: Dear all i have a basic question regarding SPR experiment and that is: What is the recommended glycerol concentration in the protein sample for doing SPR experiment and getting the best possible result.? i have kept 10% Glycerol in my protein preparation..is it O.K ? Thanx in advance -- Regards Faisal School of Life Sciences JNU
