Hi Frank
If you get crystals in high conc. of PEG400, (30-40%) DDM has a tendency
to form detergent crystals.
Try to run the the entire drop on an SDS gel, if you do not see your
protein band anymore, keep looking for crystal conditions
cheers
Preben
On 5/16/13 10:07 PM, Yibin Lin wrote:
Hi fork,
I am working with a membrane protein. I got some crystals from peg400.
All of these crystals don't diffract. I tried to use a needle to break
crystal. They didn't break into small one, look like gel, or oil,
something like that. Do anybody has deal about these crystals.
Detergent crystal? My protein contains DDM.
Thanks.
Frank,
--
J. Preben Morth, Ph.D
Group Leader
Membrane Transport Group
Nordic EMBL Partnership
Centre for Molecular Medicine Norway (NCMM)
University of Oslo
P.O.Box 1137 Blindern
0318 Oslo, Norway
Email: [email protected]
Tel: +47 2284 0794
http://www.jpmorth.dk
