Rajiv, I don't quite get your idea. Once the crystals of the single proteins have grown, you can't "soak" the other protein in, can you? Or do you mean something else?
Umri, if you do get crystals of one of the components it's well worth trying "cross-seeding" into the complex, again using random screens. There are a few examples where this has worked, particularly with antibodies. Patrick On 24 May 2013 06:43, Rajiv K Bedi <[email protected]> wrote: > Dear Umri, > > I think the main problem is co-crystallization. > > What I would do is crystallize protein and antibody separately and then > soak protein crystals into reservoir solution containing antibody or vice > versa. > And do try to get crystals from different conditions which may alter the > space group and thereby improve diffraction quality, hopefully. > > All the best, > Rajiv > -- [email protected] Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
