Instead of making the crystal thicker, why don't you make the beamsize smaller
? Aka microfocus
If this is your cryo-condition then I would work on it a bit more or change the
drop ratio of protein:reservoir so that you can use a higher PEG3350
concentration in your reservoir. I would aim for >25% PEG3350.
What happens if you change the salt to something different ?
Have you tried an additive screen ?
Jürgen
On May 28, 2013, at 9:18 PM, 姜艳 wrote:
Dear professors,
I get my crystal in 0.1M Tris, PH7.5, 200mM (NH4)2SO4, and 20% PEG3350,
however, it is very thin. From one side, the diffraction is perfect, about
2.2A, but from the other side, diffraction is too bad, the spots look like a
thread! Process cannot be done by HKL2000.
As top guns of this field, could you give me some suggestion to make the
crystal thicker? I will be grateful for your kind help.
Best,
Jiang Yan
Institute of Biophysics, Chinese Academy of Sciences
Beijing, Chaoyang District
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://lupo.jhsph.edu
