Hello CCP4BBers, I am currently playing with some crystals that seem to enjoy lower temperatures, and I was thinking of breaking the 0°C threshold.
Looking for examples of this in the literature is problematic, as searching for examples in the PDB (Under the advanced search-> crystal properties-> temperature (K)) turns up a large amount of false positives. Many otherwise supremely intelligent people seem unable or unwilling to grasp the concept of Kelvin (it's amazing how many protein structures were solved only 22 degrees above absolute zero...). I was wondering if anyone has much experience in this area? I see a few structures e.g. 2Z97 (-5°C) and 4H0W (-2°C), but I was wondering if anyone has a more systematic knowledge, some more examples, and what the parameters and best practice of this technique are. Many thanks, Glenn Masson MRC-Laboratory of Molecular Biology
