Hi, Shiqing,
In my opinion, it is not good idea.
If you want to optimize the protein buffer than you could look at
"thermofluor" method of protein buffer optimization.
BTW, have you tried seeding?
03.06.2013 10:56, Qing Shi ?????:
Hi everyone,
I wonder if I can add PEG or Li2SO4 into protein sample for new
screen? Because optimization seems no good result, initial crystal
was got in Bis-Tris, PEG8000, Li2SO4. If it is OK, then what's the
concentration of PEG or Li2SO4?
Best regards,
Shiqing
--
Eugene Osipov
Junior Research Scientist
Laboratory of Enzyme Engineering
A.N. Bach Institute of Biochemistry
Russian Academy of Sciences
Leninsky pr. 33, 119071 Moscow, Russia
e-mail: [email protected]
