Hi Mirek, hi all, I'm also very interested in the topic, so please keep me up with the replies, or make sure to post a summary, please.
In addition to the price, the problem we're facing with GSH-beads from GE (although we haven't tried others yet) is that we can't manage to deplete our lysates. We are always left with a large amount of unbound GST-tagged protein in the flow through, that is eventually captured by a second, third and sometime fourth incubation with fresh beads. Using larger beads volume won't help. Has anybody faced and/or overcame this problem? Thanks a lot in advance, ciao, Sebastiano On Jun 4, 2013, at 8:54 PM, "Cygler, Miroslaw" <miroslaw.cyg...@usask.ca> wrote: > Hi, > I would like to ask the bb faithful for their experience with the glutathione > affinity resins. We have been using so far the Glutathione Sepharose fast > flow from GE but the price is getting steeper. We found Glutathione Superflow > resin from Clontech to be significantly less expensive and Glutathione > agarose from Fisher somewhere in-between. We have no experience with the > latter two resins and I wonder what is the experience of other people with > these resins? Do they have decent binding capacity? Can they be efficiently > regenerated or are they a single use only? > Thanks for your help, > > Mirek > > > -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990
