Perhaps my question was not expressed well. I wanted to know if proteins crystallize more frequently when the protein concentration is in the range 5-30mg/ml. The answer pointed out by my colleague Todd Green is on the page http://www.douglas.co.uk/PDB_data.htm
Thanks for your inputs. Debasish From: Orru, Roberto [mailto:roberto.o...@emory.edu] Sent: Monday, June 10, 2013 5:04 PM To: Debasish Chattopadhyay Subject: RE: Protein concentration for crystallization Dear Debasish, On my memory there are 2 way (but I cannot say that are the only 2!) First: if you have the structure and you know the water content, you can guess the amount of protein crystallized in your drop by calculating the volume of the crystals. Second (if you can waste your drops): Fish all the crytsals in any drop for a given concentration, load a sds page w/ silver staining developing and compare it with a calibration curve done with your same protein in the same gel. Best R. ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Debasish Chattopadhyay [debas...@uab.edu] Sent: Monday, June 10, 2013 10:49 To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Protein concentration for crystallization What would be a convenient way to estimate what percentages of proteins have been crystallized in a concentration range, for example 5-30 mg? Debasish Chattopadhyay University of Alabama at Birmingham CBSE-250 1025 18th Street South, Birmingham, Al-35294 USA Ph: (205)934-0124; Fax: (205)934-0480 ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
