-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Jiang Yan,
how many reflections to you have in the free-set. At P6422 with a resolution of 2.8 you may have only a few hundred and thus not get a reliable Rfree value. Its low value may be due to overfitting. What does the cyrstal packing look like: Do you have the impression there is room for one or two extra residues? If not and if your model looks pleasing than you may not have a problem but a well refined structure. Best, Tim On 06/26/2013 06:05 AM, 姜艳 wrote: > Hi everyone, > > > I now meet some problems when trying to solve structure.Space group > is P6422, and Mathews function shows there are 4 molecules in one > asymmetry unit. However, Phenix-autobuild shows only 2 molecules in > on one asymmetry unit, after refinement, Rfree=20%,(resolution is > 2.8A). Although AA fit the map well, there are still some green and > red density. I wonder if anyone met this problem before? Any > suggestion is welcome. > > > Best, Jiang Yan - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFRyr28UxlJ7aRr7hoRAvNFAJ9wv2Yharh75/Eo5lfMeboViVuJ8wCgljS8 dh1VqqeriYb6uM9F5hCa77M= =+0kN -----END PGP SIGNATURE-----
