Hi Peter, I won't claim to be an expert, however, there is a small textbook by Robert K. Scopes called "Protein Purification: Principles and Practice," that has very good practical and theoretical information about column chromatography (among other methods). I have found this book useful a number of times when I have had similar questions.
Good luck, Mike ----- Original Message ----- From: "Peter Hsu" <[email protected]> To: [email protected] Sent: Saturday, June 29, 2013 2:01:15 PM GMT -08:00 US/Canada Pacific Subject: [ccp4bb] off topic: good peak on gel filtration Hi all, I've generally always thought as long as the peak was symmetrical and not too broad would suggest a good sample. However, looking at my previous runs in the past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200, or slightly broader peaks with about 3mL (all symmetrical peaks, roughly similar amounts loaded on the columns). I'm curious to see what people's views are as far as what constitutes a broad peak and how much that can end up affecting crystallization of the sample. Thanks for any responses. Peter -- Michael C. Thompson Graduate Student Biochemistry & Molecular Biology Division Department of Chemistry & Biochemistry University of California, Los Angeles [email protected]
