Dear Fulvio Saccoccia As you suspected it might be I4 instead of P4.
Since fractional coordinates are (0.5, 0.5, 0.479) and, 0.479 is almost 0.5. Try to integrate and scale in I4 and see how chi-square behaves. Raj On Fri, Jun 7, 2013 at 11:17 AM, Fulvio Saccoccia < [email protected]> wrote: > Dear ccp4bb users, > I have a data set collected at 2.2A resolution that I can integrate in > P4. Aimless, Phaser and Xtriage recognized a pseudotranslation (34.6% of > origin peak) at fractional coordinates 0.500 0.500 0.479 (ORTH 46.7 > 46.7 37.4). However labelit.index run with sublattice_allow=true search > did not detect the pseudotranslation vector. So far, any attempts to phase > by molecular replacement was unsuccesfull and I suspect that it could be > due to incorrect assignement of space group: > > Now, some questions arise: > 1) does the pseudo translation exist or do not? > 2) if translational NCS is present, could it mimic a P41/P42/P43 space > group? > 3) in any case, any advices in order to properly deal with tNCS will be of > great help? > > I thank you in advance > Best wishes > > -- > Fulvio Saccoccia, PhD > Sapienza University of Rome > Dept. of Biochemical Sciences "A. Rossi Fanelli" > Piazzale Aldo Moro, 5 > 00185 Rome (Italy) >
