Dear Vivoli, Thanks for your kind inputs. The case of Proline is already taken care of during cloning. I should have pointed this, but thanks that you did mentioned it. Thanks again for inputs. With best regards Rajesh
On Sun, Jul 21, 2013 at 12:34 PM, K Singh <ksc...@gmail.com> wrote: > Dear All, > I have expressed fusion protein that is expressed in the following format > > Fusion partner - Enterokinase cleavage site - Protein of interest. > > somehow enterokinase is not doing its job though following manufacturers > recommendations. any inputs like what protocol worked. > > Many thanks for your inputs > > Kris >
