Dear all, I would like to thank everyone for being so kind and sparing their time to respond. Just to summarise from all the responses that I got that may help someone else: MBP on its own is monomeric and tends to 'magically' help pretty much every protein you throw at it to become soluble. In most cases in helps enormously to obtain stable protein. However, in a number of cases including mine, although it can "miraculously" solubilise otherwise difficult to express in soluble form proteins, you may end up with a MBP-target protein fusion that although soluble, may not be properly folded and as a result you end up with soluble but aggregated protein. This seems to be target protein dependent.
Best wishes Kostas On 24 July 2013 14:07, Konstantinos Paraskevopoulos < [email protected]> wrote: > Dear all, > I would like to ask if someone has experience with maltose binding protein > (MBP) as a tag. > My protein fused to MBP seems to form oligomers that I find difficult to > prevent and I was wondering if mbp behaves similar to gst and may also > be prone to dimerisation/oligomerisation > > Many thanks in advance > Kostas Paraskevopoulos > Research fellow > University of edinburgh >
