Dear Appu,
one direction of your cristal has very anistropic diffraction. I think
your cristal has grown like multi-layer. The problem is probably due to
the DNA. If the different "layer" of your cristal are not very well
aligned, you have this type of problem.
In my opinion, you can try to select a subset of your data in the best
direction and try to find your cell parameters and space grp. After
that, if you have high symmetry parameters it could be sufficient with
the subset, or you can try to reindex the other part of your data. I
collect a lot of this type of data set. And all the time it was
difficult to deal with. If you have time, and if your cristal has not
suffer from radiation damage, you can try smaller oscillation angle.
Hope to help you
Le 01/08/13 15:44, Appu kumar a écrit :
Dear all ccp4 user,
We have collected a data set of 370 frames with 0.5 OSC for a
protein-DNA complex on RAXIS IV detector. The spots look mostly
clustered from 50A to 6A region but the whole data is completely
spreaded to 2.8A. There are many spots which are very near to each
other as if they were merged but closely placed. When we are trying to
index the data, its not picking all the spots correctly and giving a
unit cell dimension variable from 150 to 500A in one of the axis. Rest
two axis axes of unit cell are almost similar ~ 55A, 111A. We tried
processing with HKL2000 but not able to index it., I am attaching four
4 images for every 90 degree frames collected. Please look at these
images and give your valuable input regarding indexing problem.
your suggestions and support will be highly appreciated. Please guide
me and help me sorting out the problem.
Thank you
