Dear Zhizhi, we had a case like that. I would switch to slightly different buffer (e.g. different pH) so crystals do not appear overnight, and then do crystallisation screening. I bet you will have many hits in different conditions, likely with bigger crystals. Good luck! Best wishes, Tomas
On Fri, Aug 9, 2013 at 8:31 PM, ZHIZHI WANG <[email protected]> wrote: > Hi all, > After I purified my target protein by ion exchange, I left the fractions > with high protein concentrations overnight @4C. Now I saw a lot of small > needle crystals inside the EP tubes this morning. > I wonder whether there is any technique or method to get bigger crystals from > this? > > ZZ
