Hi, I've just got a quick question about getting ligands bound to proteins in crystals. I've managed to co-crystallize my proteins with the various ligands, and I'm aware that soaking will work as well but I want to speed up the process.
Would this work? If I had a bunch of crystals that have grown in their drop, can I pipette the ligand (say 0.6ul to get a desired conc.) onto the drop and just let the crystals soak that way. Any dilution that would have occurred when then readjust as the drop will be sealed again. Perhaps the crystals would get a little stressed when adding the high concentrated ligand and this method would not work…? Thanks, Chris -- Christopher Browning, Ph. D Vertex Pharmaceuticals (Europe) Ltd 86-88 Jubilee Avenue Milton Park Abingdon Oxfordshire OX14 4RW United Kingdom Tel +44 (0) 1235 438327 [email protected] www.vrtx.com<http://www.vrtx.com/> This email message and any attachments are confidential and intended for use by the addressee(s) only. If you are not the intended recipient, please notify me immediately by replying to this message, and destroy all copies of this message and any attachments. Thank you. Vertex Pharmaceuticals (Europe) Ltd. Registered in England and Wales, company no. 2907620 Registered Office: 86 - 88 Jubilee Avenue, Milton Park, Abingdon, Oxfordshire, OX14 4RW, UK
