-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Rakesh,
did you try to integrate including the 'streaky' images? They do not look so bad. You might get away with it, especially if you have a candidate structure for molecular replacement. Try different programs and see which one performs best with your data and do not judge this from Rmerge, but from whether or not you can solve your structure! Best, Tim On 09/19/2013 04:41 PM, Rakesh Chatterjee wrote: > hello everyone, i am trying to solve a structure of a protein > (catalytic in nature). i ve got some crystal in sodium formate. > crystal have thin plate morphology and whenever i am trying to > diffract them, at certain images spot streaking is observed. so i > am trying to merge some data sets as one data set alone is not > sufficient for completeness. this results in a high Rmerge say > 0.25 along with 3.2 redundancy and completeness of 98.7 (total) and > 89.4 (last bin) with i/sigma >2 in last bin (2.59A). how can i > improve my data. datas are collected in in Cu kalpha anode. ive > tried peg 400, glycerol, ehylene glycol, sucrose and sorbitol as > cryo supplements with formate but there is no improvement. further > the datasets have high mosaicity >1 and cell parameters vary > sometime. the space goup is C121. i am using HKL2000 for scaling > and integration. i ve even tried seeding but morphology remains > the same. the protein is highly pure >95% and homogenous (mass > spectro anlysis Maldi). Any suggestions will be helpful. > > thanx in advance > > rakesh > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.14 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFSOw7hUxlJ7aRr7hoRAn64AKD1FABPJVRfmQbo4/oMUu4C2pG8sACgju45 c5rhU/ysceaqhgbueW7AdKE= =R85t -----END PGP SIGNATURE-----
