Gloria, Here is how we are now doing it, courtesy of Yu-Jing's and Merlin's fine development work. We have also tried it to quantitatively remove tags by TEV cleavage, but we haven't solved that problem yet.
Regards, Michael Tan, Yu-Jing, Wei-Han Wang, Yi Zheng, Jinlan Dong, Giovanni Stefano, Federica Brandizzi, R. Michael Garavito, Gavin E. Reid, and Merlin L. Bruening (2012) Limited Proteolysis Via Millisecond Digestions in Protease-Modified Membranes. Analytical Chemistry 84(19), 8357-63 **************************************************************** R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334 Email: [email protected] **************************************************************** On Oct 8, 2013, at 3:34 PM, Gloria Borgstahl wrote: > We are trying to apply this approach for the first time to define a > crystallizable domain of our protein of interest. Can anyone send a protocol > that includes exactly how to do the mass spectrometry measurement? Our core > lab here doesn't know and I'm just not that gifted. Happy October, G
