Thanks Robbie
I found what I was looking for in PDB_REDO.

Thanks

Mahesh


On Fri, Oct 11, 2013 at 4:49 PM, Robbie Joosten
<[email protected]>wrote:

> Hi Mahesh,
>
> They will be close but not the same. RMSDs should be very close normally,
> but different treatment of hetero compounds (ligands and such), outliers
> and
> LINKs added during annotation may cause small deviations. The deviations in
> R-factors can be much larger for all sorts of reasons. The data that is
> used
> now can be (slightly) different from what was used before due to different
> scaling, merging of I+ and I-, conversion from F to I, outlier rejection,
> R-free set annotation, deposition errors (i.e. missing data), or deposition
> of all measured data, not just the data used in the final refinement (this
> is actually a good thing). Different programs have different treatment of
> bulk solvent. There can be rounding errors is the conversion from TLS to
> anisotropic B-factors. The use of riding hydrogens can also make a big
> difference.
> There are several papers that discuss the sources of these R-factor
> deviations, e.g. papers about EDS, PDB_REDO and phenix.model_vs_data.
>
> Cheers,
> Robbie
>
> > -----Original Message-----
> > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of
> > Mahesh Lingaraju
> > Sent: Friday, October 11, 2013 21:59
> > To: [email protected]
> > Subject: [ccp4bb] R and R free from CIF file deposited in PDB
> >
> > Hello Experts,
> >
> > I was wondering if the R, Rfree, RMSD for bonds and angles calculated by
> > polygon plug in in PHENIX GUI using the cif file & pdb file deposited in
> protein
> > structure database would be the same as reported in database/publication
> ?
> >
> > I apologize in advance if this is a PHENIX specific question.
> >
> > Many thanks
> >
> > Mahesh
>

Reply via email to